Single molecule immuno-detection technology is a digitalized immune-assay. It achieves signal recognition and capture at a single molecule level that to be tested through special signal detection methods, and marks all detection units in the detection system with "0" and "1" signal labeling methods. By calculating the proportion or number of signal units of "0" and "1", ultra sensitive and quantitative analysis can be easy to achieved.

Compared with traditional immune-detection technology, single molecule assay achieves a sensitivity increase of 1000x at 1fg/ml level. With the digitalized immune signal output, making it the most sensitive immune-detection technology at present.